Abstract
Plants survive by deploying appropriate stress responses under rapidly changing environmental conditions. Vascular plants transmit environmental information among distant organs. Long-distance signaling plays a crucial role in plant adaptation and subsequent survival under severe environmental conditions. In model plants, the micrografting method has recently emerged as an important method to elucidate tissue-to-tissue communication via hormones, RNAs, and peptides. In this chapter, I describe a micrografting method for Arabidopsis thaliana to graft shoots onto roots of plants with different genotypes. This grafting method may facilitate further research investigating how vascular plants integrate environmental information among distant organs via long-distance signaling.
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Acknowledgments
This work was supported by JSPS KAKENHI Grant Number JP19H03255 (to F.T.), JP20K21437 (to F.T.) and by research grants from the Toray Science Foundation (to F.T.). I appreciate the valuable comments of Dr. Kazuo Shinozaki of the RIKEN Center for Sustainable Resource Science.
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Takahashi, F. (2022). Use of Micrografting to Study the Role Played by Peptide Signals in ABA Biosynthesis. In: Yoshida, T. (eds) Abscisic Acid. Methods in Molecular Biology, vol 2462. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2156-1_8
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DOI: https://doi.org/10.1007/978-1-0716-2156-1_8
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