Abstract
The chicken embryonated egg is one of the most versatile and widely used systems for the isolation and propagation of avian viruses, including infectious bronchitis virus. Fresh tissue samples including trachea, cecal tonsils, and kidney are the most used samples for IBV isolation, although oropharyngeal or tracheal swabs can also be used. This virus can be isolated by inoculation into the allantoic cavity. Characteristic embryonic lesions include stunting and curling of the embryo and its feet. Between 48 h and 72 h postinoculation, inoculated eggs can be screened to detect the presence of infectious bronchitis and hemagglutinating viruses (such as Newcastle disease or avian influenza viruses) by rapid plate hemagglutination assay; IBV can acquire hemagglutinating capabilities by treating the allantoic fluids with Clostridium perfringens neuraminidase. Samples that after being inoculated into embryonated eggs and yield positive hemagglutination after neuraminidase treatment and/or induce characteristic embryonic lesion are positive for IBV isolation, and their allantoic fluids can be confirmed and genotyped by molecular methodologies.
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Banda, A., Yan, L. (2022). Isolation and Propagation of Infectious Bronchitis Virus (Avian Coronavirus) in Chicken Embryonated Eggs. In: Wang, L. (eds) Animal Coronaviruses. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2091-5_7
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DOI: https://doi.org/10.1007/978-1-0716-2091-5_7
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