Abstract
Autophagy is an intracellular degradation process that maintains the cellular homeostasis and it is regulated in multiple ways, both in health and disease. Assessment of autophagic flux in cells is an important approach for understanding the function of autophagy in biological contexts. Here, we describe a new tool for the qualitative and quantitative determination of autophagic flux using a dual lentiviral reporter system that generates a fusion HiBiT-GFP-LC3B protein suitable for generating stable cell lines.
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Acknowledgments
This work was supported by a German Research Foundation (DFG) grant (VE1153/1-1) and a Josepha und Charlotte von Siebold Habilitandinnen-Förderprogramm (University of Duisburg-Essen) to S.V.R. This project has received additional funding from the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie Grant Agreement No. 798637 and a grant from DFG (PE 2696/1-1) to S.P.-L. Graphical art in Fig. 4 was partially prepared using Smart Servier Medical Art (https://smart.servier.com/) by Servier (Suresnes, France) licensed under a Creative Commons Attribution 3.0 Unported License (https://creativecommons.org/licenses/by/3.0/).
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Will, R. et al. (2022). A Dual HiBiT-GFP-LC3 Lentiviral Reporter for Autophagy Flux Assessment. In: Norberg, H., Norberg, E. (eds) Autophagy and Cancer. Methods in Molecular Biology, vol 2445. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2071-7_6
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DOI: https://doi.org/10.1007/978-1-0716-2071-7_6
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-2071-7
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