Abstract
The development of new blood and lymphatic vessels, through the process of angiogenesis and lymphangiogenesis, respectively, is critical to the development and growth of tumors, and integral to the process of metastasis. Lymphatic vessel density can be assessed as a surrogate measure of lymphangiogenesis in human tissue samples. Lymphatic vessel density has been shown to be associated with lymph node metastasis and patient survival in various solid tumor types. Here we describe a method for quantifying the number of lymphatic vessels within tumor tissue that can also be used to assess lymphatic vessel invasion, and compare with blood vessel density and invasion.
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References
Christiansen A, Detmar M (2011) Lymphangiogenesis and cancer. Genes Cancer 2:1146–1158
Mohammed RA, Ellis IO, Elsheikh S, Paish EC, Martin SG (2009) Lymphatic and angiogenic characteristics in breast cancer: morphometric analysis and prognostic implications. Breast Cancer Res Treat 113:261–273
Mohammed RA, Ellis IO, Mahmmod AM, Hawkes EC, Green AR, Rakha EA, Martin SG (2011) Lymphatic and blood vessels in basal and triple-negative breast cancers: characteristics and prognostic significance. Mod Pathol 24:774–785
Mohammed RA, Martin SG, Mahmmod AM, Macmillan RD, Green AR, Paish EC, Ellis IO (2011) Objective assessment of lymphatic and blood vascular invasion in lymph node-negative breast carcinoma: findings from a large case series with long-term follow-up. J Pathol 223:358–365
Schacht V, Dadras SS, Johnson LA, Jackson DG, Hong YK, Detmar M (2005) Up-regulation of the lymphatic marker podoplanin, a mucin-type transmembrane glycoprotein, in human squamous cell carcinomas and germ cell tumors. Am J Pathol 166:913–921
Evangelou E, Kyzas PA, Trikalinos TA (2005) Comparison of the diagnostic accuracy of lymphatic endothelium markers: Bayesian approach. Mod Pathol 18:1490–1497
Storr SJ, Safuan S, Mitra A, Elliott F, Walker C, Vasko MJ, Ho B, Cook M, Mohammed RA, Patel PM, Ellis IO, Newton-Bishop JA, Martin SG (2012) Objective assessment of blood and lymphatic vessel invasion and association with macrophage infiltration in cutaneous melanoma. Mod Pathol 25:493–504
Zhang S, Zhang D, Yi S, Gong M, Lu C, Cai Y, Tang X, Zou L (2017) The relationship of lymphatic vessel density, lymphovascular invasion, and lymph node metastasis in breast cancer: a systematic review and meta-analysis. Oncotarget 8:2863–2873
Zhang S, Yi S, Zhang D, Gong M, Cai Y, Zou L (2017) Intratumoral and peritumoral lymphatic vessel density both correlate with lymph node metastasis in breast cancer. Sci Rep 7:40364
Liang L, Huang WT, He RQ, Liang HW, Huang CQ, Zhou H, Wei FL, Zhou SS, Peng ZG, Chen G, Chen JQ, Qin XG (2018) A meta-analysis of the lymphatic microvessel density and survival in gastric cancer with 1809 cases. Oncotarget 9:5406–5415
Vermeulen PB, Gasparini G, Fox SB, Colpaert C, Marson LP, Gion M, Belien JA, de Waal RM, Van Marck E, Magnani E, Weidner N, Harris AL, Dirix LY (2002) Second international consensus on the methodology and criteria of evaluation of angiogenesis quantification in solid human tumours. Eur J Cancer 38:1564–1579
Ahmad NS, Martin SG, Storr SJ (2016) Immunohistochemical assessment of leukocyte involvement in angiogenesis. Methods Mol Biol 1430:49–57
Acknowledgments
S.J.S. is funded by the University of Nottingham Research Vision.
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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
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Martin, S.G., Rakha, E., Storr, S.J. (2022). Quantifying Lymphatic Vessel Density in Human Tissue Samples. In: Benest, A.V. (eds) Angiogenesis. Methods in Molecular Biology, vol 2441. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2059-5_14
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DOI: https://doi.org/10.1007/978-1-0716-2059-5_14
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