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Single-Unit Electrophysiological Recordings of Primary Muscle Sensory Neurons Using a Novel Ex Vivo Preparation

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Contemporary Approaches to the Study of Pain

Part of the book series: Neuromethods ((NM,volume 178))

Abstract

For decades, multiple electrophysiological techniques have been used to characterize the responses of primary afferents to a variety of stimuli under both normal and pathological conditions. With these strategies, experimenters are able to quantify afferent response properties, such as threshold and firing rates, and determine the functional classifications of individual cells. New research techniques have been developed that allow for both characterization of the afferent responses and correlation of the physiology and neurochemical or molecular identity, providing a comprehensive phenotype of the sensory neurons being analyzed. Dual characterization provides insight about the role of specific markers expressed in primary sensory neurons and how they may influence sensory transduction. In this chapter, we describe a powerful electrophysiological technique that allows for ex vivo recordings from intact primary muscle afferents from two target muscle groups, similar to that previously described for cutaneous afferents.

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Acknowledgments

This work was supported by grants to MPJ from the NIH (R01AR064551 and R01NS113965).

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Authors and Affiliations

  1. Division of Pain Management, Department of Anesthesia, Cincinnati Children’s Medical Center, Cincinnati, OH, USA

    Luis F. Queme & Michael P. Jankowski

  2. Department of Pediatrics, College of Medicine, University of Cincinnati, Cincinnati, OH, USA

    Michael P. Jankowski

Authors
  1. Luis F. Queme
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  2. Michael P. Jankowski
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Corresponding author

Correspondence to Michael P. Jankowski .

Editor information

Editors and Affiliations

  1. Department of Neurobiology, Pittsburgh Center for Pain Research, University of Pittsburgh, Pittsburgh, PA, USA

    Rebecca P. Seal

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Queme, L.F., Jankowski, M.P. (2022). Single-Unit Electrophysiological Recordings of Primary Muscle Sensory Neurons Using a Novel Ex Vivo Preparation. In: Seal, R.P. (eds) Contemporary Approaches to the Study of Pain. Neuromethods, vol 178. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2039-7_6

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  • DOI: https://doi.org/10.1007/978-1-0716-2039-7_6

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2038-0

  • Online ISBN: 978-1-0716-2039-7

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