Abstract
Single-cell proteomics is a novel application area of bioanalysis aiming to characterize proteomes of isolated single cells, which in contrast to bulk cell analysis has the potential to reveal a more detailed heterogeneity of cell populations. Although several antibody-based targeted approaches have been readily available for single-cell analysis, so far only the mass spectrometry methodology can offer unbiased proteome profiling. While this strategy has only recently emerged, it has already demonstrated unparalleled analytical power quantifying >1000 proteins in single cells. Several applications of a general isobaric labeling scheme for multiplexed sample preparation and data acquisition have been outlined using various cell types and instrumentation. This chapter provides a typical example of mass spectrometry-based single-cell proteomics workflow with details about the critical steps of analysis and alternative methods useful for optimization purposes.
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Acknowledgments
This work was supported by the Swedish Research Council (grant 2018-06156, VR).
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Végvári, Á. (2022). Mass Spectrometry-Based Analytical Strategy for Single-Cell Proteomics. In: Corrales, F.J., Paradela, A., Marcilla, M. (eds) Clinical Proteomics. Methods in Molecular Biology, vol 2420. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1936-0_6
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DOI: https://doi.org/10.1007/978-1-0716-1936-0_6
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