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Expression Profiles of Estrogen-Regulated MicroRNAs in Cancer Cells

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Estrogen Receptors

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2418))

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Abstract

MicroRNAs play critical roles through their impact on posttranscriptional gene regulation. In cancer, they can act as oncogenes or tumor suppressors and can also function as biomarkers. Here, we describe a method for robust characterization of estrogen-regulated microRNA profiles. The activity of estrogen is mediated by two nuclear receptors, estrogen receptor alpha and estrogen receptor beta, and a transmembrane G-protein coupled estrogen receptor 1. This chapter details how to prepare cells for optimal estrogen response, directions for estrogen treatment, RNA extraction, different microRNA profiling approaches, and subsequent confirmations.

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Acknowledgments

This work was supported by Swedish Cancer Society (Cancerfonden CAN 2018/596 to C.W.), the Stockholm County Council (Region Stockholm, HMT 2020-0346 to C.W.), the Swedish Research Council (2017-01658 to C.W.; 2019–05165 to C.K.), Knut & Alice Wallenberg foundation (KAW 2016.0174 to C.K.), Ruth & Richard Julin foundation (2020-00294 to C.K.), and Lillian Sagen & Curt Ericsson research foundation (2021-00427, C.K.).

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Authors and Affiliations

  1. SciLifeLab, Department of Protein Science, KTH—Royal Institute of Technology, Solna, Sweden

    Amena Archer & Cecilia Williams

  2. Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden

    Amena Archer & Cecilia Williams

  3. SciLifeLab, Department of Microbiology, Tumor and Cell biology, Karolinska Institutet, Solna, Sweden

    Claudia Kutter

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  1. Amena Archer
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  2. Claudia Kutter
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  3. Cecilia Williams
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Correspondence to Cecilia Williams .

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  1. Sanford School of Medicine, University of South Dakota, Vermillion, SD, USA

    Kathleen M. Eyster

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Archer, A., Kutter, C., Williams, C. (2022). Expression Profiles of Estrogen-Regulated MicroRNAs in Cancer Cells. In: Eyster, K.M. (eds) Estrogen Receptors. Methods in Molecular Biology, vol 2418. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1920-9_18

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  • DOI: https://doi.org/10.1007/978-1-0716-1920-9_18

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