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Retroviral Transduction of NKT Hybridoma Cells

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Invariant Natural Killer T-Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2388))

Abstract

Natural killer T (NKT) cells have been shown to bridge innate and adaptive immunity. However, the rare population and hard-to-transfect of primary NKT cells slow down our understanding of cellular and molecular mechanisms of NKT development and function. To overcome these drawbacks, NKT hybridomas, especially DN32.D3 cells, are applied to study NKT cells in vitro and becoming a valuable tool. Here, we describe the method in the genetic manipulation of DN32.D3 cells by retrovirus, including the generation and concentration of retrovirus, retroviral transduction of DN32.D3 cells, and evaluation of transduction efficiency.

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Acknowledgments

The authors thank Dr. Li Bai at the University of Science and Technology of China for the DN32.D3 hybridomas and Dr. Zhongjun Dong at Tsinghua University for the pCL-Eco and pMSCV-ubc-EGFP vectors. This work was supported by grants from the National Key Research and Development Program of China (2017YFA0104500), the National Natural Science Foundation of China (32070897, 31671244, 31872734), the Foundation for Innovative Research Groups of the National Natural Science Foundation of China (81621001), Beijing Natural Science Foundation (7202079), and the Non-Profit Central Research Institute Fund of Chinese Academy of Medical Sciences, 2019PT320006.

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Authors and Affiliations

  1. Department of Immunology, School of Basic Medical Sciences, Peking University, NHC Key Laboratory of Medical Immunology (Peking University), Beijing, China

    Ke Wang, Rong Jin & Qing Ge

  2. Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing, China

    Ke Wang & Qing Ge

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  1. Ke Wang
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  2. Rong Jin
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  3. Qing Ge
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Correspondence to Qing Ge .

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Editors and Affiliations

  1. Department of Pathogen Biology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China

    Chaohong Liu

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Wang, K., Jin, R., Ge, Q. (2021). Retroviral Transduction of NKT Hybridoma Cells. In: Liu, C. (eds) Invariant Natural Killer T-Cells. Methods in Molecular Biology, vol 2388. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1775-5_3

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  • DOI: https://doi.org/10.1007/978-1-0716-1775-5_3

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1774-8

  • Online ISBN: 978-1-0716-1775-5

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