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Screening of Actinobacteria for Biosurfactant Production

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Methods in Actinobacteriology

Abstract

The protocol is based on screening actinomycete strains for utilizing carbon as a sole source for extracellular biosurfactant activity by a drop-collapse process. The strains were selected based on generating biosurfactants. The hemolytic activity of actinobacteria strains were ranged approximately 8–20 mm, by the drop-collapsing method and by the oil-spreading method respectively. Emulsification activity and reduced surface tension were present in the cell-free culture broth further the surface tension values observed with manufacture biosurfactants on a larger scale.

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References

  1. Liu G, Zhong H, Yang X, Liu Y, Shao B, Liu Z (2018) Advances in applications of rhamnolipidsbiosurfactant in environmental remediation: a review. Biotechnol Bioeng 115(4):796–814

    Article  CAS  Google Scholar 

  2. Kubicki S, Bollinger A, Katzke N, Jaeger KE, Loeschcke A, Thies S (2019) Marine biosurfactants: biosynthesis, structural diversity and biotechnological applications. Mar Drugs 17(7):408

    Article  CAS  Google Scholar 

  3. Franzetti A, Tamburini E, Banat IM (2010) Applications of biological surface active compounds in remediation technologies. Biosurfactants:121–134

    Google Scholar 

  4. Kralova I, Sjöblom J (2009) Surfactants used in food industry: a review. J Dispers Sci Technol 30(9):1363–1383

    Article  CAS  Google Scholar 

  5. Adu SA, Naughton PJ, Marchant R, Banat IM (2020) Microbial biosurfactants in cosmetic and personal skincare pharmaceutical formulations. Pharmaceutics 12(11):1099

    Article  CAS  Google Scholar 

  6. Thavasi R, Banat IM (2019) Introduction to microbial biosurfactants. In: Microbial biosurfactants and their environmental and industrial applications. CRC Press, Boca Raton, FL, pp 1–15

    Google Scholar 

  7. Maier RM (2003) Biosurfactants: evolution and diversity in bacteria. Adv Appl Microbiol 52:101–122

    Article  CAS  Google Scholar 

  8. Willumsen PA, Karlson U (1996) Screening of bacteria, isolated from PAH-contaminated soils, for production of biosurfactants and bioemulsifiers. Biodegradation 7(5):415–423

    Article  CAS  Google Scholar 

  9. Nitschke M, Costa SGVAO (2007) Biosurfactants in food industry. Trends Food Sci Technol 18(5):252–259

    Article  CAS  Google Scholar 

  10. Lamilla C, Braga D, Castro R et al (2018) Streptomyces luridus So3.2 from Antarctic soilas a novel producer of compounds with bioemulsificationpotential. PLoS One 13(4):e0196054

    Article  Google Scholar 

  11. Alvarez HM, Steinbüchel A (2019) Biology of triacylglycerol accumulation by Rhodococcus. In: Biology of Rhodococcus. Springer, Cham, pp 299–332

    Chapter  Google Scholar 

  12. Kügler JH, Roes-Hill L, Syldatk C, Hausmann R (2015) Surfactants tailored by the class Actinobacteria. Front Microbiol 6:212

    PubMed  PubMed Central  Google Scholar 

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Chelliah, R., Banan-Mwine Daliri, E., Oh, DH. (2022). Screening of Actinobacteria for Biosurfactant Production. In: Dharumadurai, D. (eds) Methods in Actinobacteriology. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1728-1_95

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  • DOI: https://doi.org/10.1007/978-1-0716-1728-1_95

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1727-4

  • Online ISBN: 978-1-0716-1728-1

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