Abstract
The systematic design and construction of customized protein microarrays are critical for the further successful screening of biological samples in biomedical research projects. In general protein microarrays are classified according to the content, detection method, and printing methodology, among others. Here, we are focused on the type of printing: contact and noncontact. Both approaches have advantages and disadvantages; however, in any of the approaches, a prior well design and systematic preparation of materials and/or instruments required for the customized antibody arrays is critical. In this chapter, the process for an antibody microarray by a noncontact printer is described in detail from the preparation of array content to the analysis, including quality control steps.
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Acknowledgments
We thank Raul Manzano-Roman for technical support. We gratefully acknowledge financial support from the Spanish Health Institute Carlos III (ISCIII) for the grants: FIS PI14/01538, FIS PI17/01930, and CB16/12/00400. We also acknowledge Fondos FEDER (EU) and Junta Castilla-León (COV20EDU/00187), Fundación Solórzano FS/38-2017. The Proteomics Unit belongs to ProteoRed, PRB3-ISCIII, supported by grant PT17/0019/0023, of the PE I + D + I 2017–2020, funded by ISCIII and FEDER. A. Landeira-Viñuela is supported by VIII Centenario-USAL PhD Program, and P. Juanes-Velasco is supported by JCYL PhD Program and scholarship JCYL-EDU/601/2020.
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Juanes-Velasco, P., Landeira-Viñuela, A., Hernandez, AP., Fuentes, M. (2021). Systematic and Rational Design of Protein Arrays in Noncontact Printers: Pipeline and Critical Aspects. In: Barderas, R., LaBaer, J., Srivastava, S. (eds) Protein Microarrays for Disease Analysis. Methods in Molecular Biology, vol 2344. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1562-1_2
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DOI: https://doi.org/10.1007/978-1-0716-1562-1_2
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