Abstract
Heart failure is caused by a complicated pathogenic process and has a poor prognosis. Quality of life is often impaired due to repeated hospitalization. Integrative analysis of the morphological, physiological, and molecular profiles of cardiomyocytes, which are responsible mainly for heart contraction, may lead to a deeper understanding of the pathogenesis of heart failure. However, unlike other types of cells, cardiomyocytes are relatively large, vulnerable to stress, and difficult to use for single-cell analysis. With some ingenuity, we have established a single-cardiomyocyte analysis pipeline. Here, we describe the procedure for single-cell RNA sequencing of adult mouse cardiomyocytes from isolation to analysis.
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Acknowledgments
This work was supported by Grant-in-Aid for Young Scientists from the Japan Society for the Promotion of Science (to M.K.), Grant-in-Aid for Scientific Research (B) (to S.N.), Grant-in-Aid for Scientific Research (A) from the Japan Society for the Promotion of Science (to I.K.), and grants from the Japan Foundation for Applied Enzymology (to S.N.), the SENSHIN Medical Research Foundation (to S.N.), the KANAE Foundation for the Promotion of Medical Science (to S.N.), MSD Life Science Foundation (to S.N.), The Tokyo Biomedical Research Foundation (to S.N.), Astellas Foundation for Research on Metabolic Disorders (to S.N.), The Novartis Foundation (Japan) for the Promotion of Science (to S.N.), the Japanese Circulation Society (to S.N.), Takeda Science Foundation (to S.N.), and AMED (JP20gm0810013, JP20ek0109440, JP20ek0109487, JP20ek0109406, JP20km0405209, JP20bm0704026, JP20gm6210010, JP20ek0210141, JP20ek0210152, JP19bm0804010) (to S.N. and I.K.). The authors report that they have no relationships relevant to the contents of this chapter to disclose.
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Katoh, M., Nomura, S., Yamada, S., Aburatani, H., Komuro, I. (2021). Single-Cardiomyocyte RNA Sequencing to Dissect the Molecular Pathophysiology of the Heart. In: Yoshida, Y. (eds) Pluripotent Stem-Cell Derived Cardiomyocytes. Methods in Molecular Biology, vol 2320. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1484-6_18
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DOI: https://doi.org/10.1007/978-1-0716-1484-6_18
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