Abstract
Single-cell engineering via virus based genetic manipulation allows the possibility of understanding of complex tissues. However, current delivery methods for the genetic engineering of single cells via viral transduction suffer from limitations that restrict their application. Here I present a protocol describing a precise technique which can be used for the targeted virus infection of single cells in a monolayer of cells that is optically accessible. The protocol, demonstrated here by stamping cultured Hela cells with lentiviruses (LVs), completes in a few minutes and allows stable transgene expression within a few days, at success rates approaching 80%.
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Acknowledgments
This study was supported by a Burroughs Wellcome Fund grant (ID #1018793 to R.S.)
Competing financial interests: The author declares competing financial interests. The author applied for a patent related to the virus stamping approach.
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Schubert, R. (2021). Magnetically Single-Cell Virus Stamping. In: Kojima, R. (eds) Mammalian Cell Engineering. Methods in Molecular Biology, vol 2312. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1441-9_20
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DOI: https://doi.org/10.1007/978-1-0716-1441-9_20
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1440-2
Online ISBN: 978-1-0716-1441-9
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