Abstract
Bone marrow stromal cells (BMSCs) account for an extremely small percentage of total bone marrow cells; therefore, it is technically challenging to harvest a good quantity of BMSCs with good viability using fluorescence-activated cell sorting (FACS). Here, we describe the methods to effectively isolate BMSCs for flow cytometry analyses and subsequent FACS. Use of transgenic reporter lines facilitates FACS-based isolation of BMSCs, aiding to uncover fundamental characteristics of these diverse cell populations.
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Acknowledgments
This research was supported by grants from National Institute of Health (R01DE026666 to N.O., R01DE029181 to W.O.), and the Japan Society for the Promotion of Science KAKENHI Grant JP19K19236 to Y.M. We thank for K. Mizuhashi for inventing innovative approaches described in this chapter.
Declaration of interests: The authors declare no competing interests.
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Matsushita, Y., Ono, W., Ono, N. (2021). Flow Cytometry-Based Analysis of the Mouse Bone Marrow Stromal and Perivascular Compartment. In: Espéli, M., Balabanian, K. (eds) Bone Marrow Environment. Methods in Molecular Biology, vol 2308. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1425-9_7
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DOI: https://doi.org/10.1007/978-1-0716-1425-9_7
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