Abstract
In this chapter, we describe the preparatory and spectroscopic procedures for conducting solid-state NMR experiments on microtubules (MTs) obtained from human cells and their complexes with microtubule-associated proteins (MAPs). Next to labeling and functional assembly of MTs and MT-MAP complexes, we discuss solid-state NMR approaches, including fast MAS and hyperpolarization methods that can be used to examine these systems. Such studies can provide novel insight into the dynamic properties of MTs and MT-MAP complexes.
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Acknowledgments
We thank Gert Folkers for helpful discussions and Johan van der Zwan for excellent technical support. This work was supported by the Dutch Science Foundation NWO (VENI grant 722.016.002 to SX, NWO-Groot grant 175.010.2009.002, and TOP-PUNT grant to MB) and by uNMR-NL, the National Roadmap Large-Scale NMR Facility of the Netherlands (grant 184.032.207). C.J. is supported by the grants ANR-10-IDEX-0001-02 PSL, ANR-11-LBX-0038, FRM DEQ20170336756. S.B. was supported by the FRM grant FDT201805005465, and CEFIPRA 5703-1, and J.A.S. by the European Union’s Horizon 2020 Marie Skłodowska-Curie grant agreement No. 675737, and the FRM grant FDT201904008210.
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Luo, Y. et al. (2021). Solid-State NMR Spectroscopy for Studying Microtubules and Microtubule-Associated Proteins. In: Owens, R.J. (eds) Structural Proteomics. Methods in Molecular Biology, vol 2305. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1406-8_10
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DOI: https://doi.org/10.1007/978-1-0716-1406-8_10
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