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Advances in Confocal Microscopy and Selected Applications

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Confocal Microscopy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2304))

Abstract

Over the last 30 years, confocal microscopy has emerged as a primary tool for biological investigation across many disciplines. The simplicity of use and widespread accessibility of confocal microscopy ensure that it will have a prominent place in biological imaging for many years to come, even with the recent advances in light sheet and field synthesis microscopy. Since these more advanced technologies still require significant expertise to effectively implement and carry through to analysis, confocal microscopy-based approaches still remain the easiest way for biologists with minimal imaging experience to address fundamental questions about how their systems are arranged through space and time. In this review, we discuss a number of advanced applications of confocal microscopy for probing the spatiotemporal dynamics of biological systems.

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Acknowledgments

We thank Erin O’Shea and Jennifer Lippincott-Schwartz for support and funding, and members of both labs for comments and suggestions regarding the chapter. We are especially thankful for Andy Moore and Carolyn Ott for critical reading of the manuscript.

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Authors and Affiliations

  1. Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA

    W. Matt Reilly & Christopher J. Obara

  2. Department of Molecular & Cellular Biology, Harvard University, Cambridge, MA, USA

    W. Matt Reilly

Authors
  1. W. Matt Reilly
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  2. Christopher J. Obara
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Corresponding author

Correspondence to Christopher J. Obara .

Editor information

Editors and Affiliations

  1. NIAID, National Institutes of Health, Rockville, MD, USA

    Joseph Brzostowski

  2. NIAID, National Institutes of Health, Rockville, MD, USA

    Haewon Sohn

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© 2021 This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply and Springer Nature US

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Reilly, W.M., Obara, C.J. (2021). Advances in Confocal Microscopy and Selected Applications. In: Brzostowski, J., Sohn, H. (eds) Confocal Microscopy. Methods in Molecular Biology, vol 2304. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1402-0_1

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  • DOI: https://doi.org/10.1007/978-1-0716-1402-0_1

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1401-3

  • Online ISBN: 978-1-0716-1402-0

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