Abstract
The red (R)/far-red (FR) light absorbing phytochromes are one of the major photoreceptor classes in plants. Phytochromes exist in two distinct but interconvertible forms: the R light-absorbing Pr form and the FR light-absorbing Pfr form. Upon photoactivation by light, phytochromes physically interact with their partners to transduce the light signal. Co-immunoprecipitation (Co-IP) is one of the most efficient techniques to study these protein-protein interactions in vivo. However, the co-IP procedure for phytochromes needs to be modified to allow their formation of Pr or Pfr form. Here, we describe a detailed co-IP procedure to examine which form of phytochrome (Pr or Pfr) is preferentially associated with their interacting partners in vivo.
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Acknowledgments
This work was supported by grants from the National Natural Science Foundation of China (31970262 and 31770321) and the Recruitment Program of Global Youth Experts of China to J.L.
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Song, P., Zhang, S., Li, J. (2021). Co-immunoprecipitation Assays to Detect In Vivo Association of Phytochromes with Their Interacting Partners. In: Yin, R., Li, L., Zuo, K. (eds) Plant Photomorphogenesis. Methods in Molecular Biology, vol 2297. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1370-2_8
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DOI: https://doi.org/10.1007/978-1-0716-1370-2_8
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