Abstract
Co-immunoprecipitation (CoIP) assay has been used as a powerful and routine technique to detect in vivo protein-protein interactions. Not only can it probe stable interactions, but also it is applicable for semiquantitative and inducible protein associations. Here we describe the protocol for detecting blue light-dependent protein interactions, particularly for blue light receptor cryptochrome-mediated complex formation. In addition, we present some notes which may be helpful for common Co-IP study as well.
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Acknowledgments
We thank Dr. Danmeng Zhu and Dr. Yuqiu Wang from Peking University, China for valuable advising on CoIP assay.
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Zhang, J., He, S. (2021). Co-immunoprecipitation Assay for Blue Light-Dependent Protein Interactions in Plants. In: Yin, R., Li, L., Zuo, K. (eds) Plant Photomorphogenesis. Methods in Molecular Biology, vol 2297. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1370-2_14
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DOI: https://doi.org/10.1007/978-1-0716-1370-2_14
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