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High-Throughput Doubled Haploid Production for Indica Rice Breeding

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Doubled Haploid Technology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2287))

Abstract

Anther culture is an important biotechnological tool for quick recovery of fixed breeding lines with unique gene combinations that might otherwise disappear in the course of an extended series of segregating generations in conventional breeding methods in rice. The haploid microspores in culture or the resultant haploid plants are converted to doubled haploids (homozygotes). Variation in doubled haploid lines from F1 hybrids is due to the recovery of rare gene combinations by single round of recombination following meiosis. Androgenesis in rice is largely species- and genotype-specific. O. glaberrima responds better to anther culture than O. sativa; and japonica sub-group is more responsive to microspore embryogenesis than indica types. The author provides a detailed protocol of the anther culture technique for doubled haploid production in indica rice hybrids amenable for genetic improvement.

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Acknowledgements

The author is grateful to all researchers for their valuable contributions included in this pursuit.

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Authors and Affiliations

  1. Department of Agricultural Biotechnology, College of Agriculture, OUAT, Bhubaneswar, India

    Swapan K. Tripathy

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  1. Swapan K. Tripathy
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Editors and Affiliations

  1. CPI, COMAV - Univ Politecnica Valencia, Valencia, Valencia, Spain

    Jose M. Segui-Simarro

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Tripathy, S.K. (2021). High-Throughput Doubled Haploid Production for Indica Rice Breeding. In: Segui-Simarro, J.M. (eds) Doubled Haploid Technology. Methods in Molecular Biology, vol 2287. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1315-3_20

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  • DOI: https://doi.org/10.1007/978-1-0716-1315-3_20

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1314-6

  • Online ISBN: 978-1-0716-1315-3

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