Abstract
Understanding protein-protein interactions is key to unraveling protein function in vivo. Here we describe a dual/triple-plasmid system that enables co-expression of two, or three, recombinant proteins harboring different affinity tags in the same Escherichia coli cell. This novel protein expression system provides a platform to understand protein-protein interactions and enables researchers to study protein complex formation and in vivo localization.
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Yu, C., Bianco, P.R. (2021). In Vivo Binding of Single-Stranded DNA-Binding Protein to Stalled Replication Fork Helicases. In: Oliveira, M.T. (eds) Single Stranded DNA Binding Proteins. Methods in Molecular Biology, vol 2281. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1290-3_5
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DOI: https://doi.org/10.1007/978-1-0716-1290-3_5
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