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Fetal Growth Plate Cartilage : Histological and Immunohistochemical Techniques

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Chondrocytes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2245))

Abstract

Skeletal development is a tightly regulated process that primarily occurs through two distinct mechanisms. In intramembranous ossification, mesenchymal progenitors condense and transdifferentiate directly into osteoblasts, giving rise to the flat bones of the skull. The majority of the skeleton develops through endochondral ossification, in which mesenchymal progenitors give rise to a cartilaginous template that is gradually replaced by bone. The study of these processes necessitates a suitable animal model, a requirement to which the mouse is admirably suited. Their rapid reproductive ability, developmental and physiologic similarity to humans, and easily manipulated genetics all contribute to their widespread use. Outlined here are the most common histological and immunohistochemical techniques utilized in our laboratory for the isolation and analysis of specimens from the developing murine skeleton.

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Authors and Affiliations

  1. Departments of Orthopedic Surgery, Medicine and Cell and Developmental Biology, University of Michigan School of Medicine, Ann Arbor, MI, USA

    Zachary Tata, Christophe Merceron & Ernestina Schipani

Authors
  1. Zachary Tata
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  2. Christophe Merceron
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  3. Ernestina Schipani
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Correspondence to Ernestina Schipani .

Editor information

Editors and Affiliations

  1. Department of Anatomy and Neurobiology, Northeast Ohio Medical University, Rootstown, OH, USA

    Tariq M. Haqqi

  2. Department of Surgery, The Children's Hospital of Philadelphia, Philadelphia, PA, USA

    Véronique Lefebvre

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Tata, Z., Merceron, C., Schipani, E. (2021). Fetal Growth Plate Cartilage : Histological and Immunohistochemical Techniques. In: Haqqi, T.M., Lefebvre, V. (eds) Chondrocytes. Methods in Molecular Biology, vol 2245. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1119-7_5

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  • DOI: https://doi.org/10.1007/978-1-0716-1119-7_5

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1118-0

  • Online ISBN: 978-1-0716-1119-7

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