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Techniques for Characterizing Cytomegalovirus-Encoded miRNAs

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Human Cytomegaloviruses

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2244))

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Abstract

microRNAs (miRNAs) are small noncoding RNAs that regulate gene expression at the posttranscriptional level by binding to sites within the 3′ untranslated regions of messenger RNA (mRNA) transcripts. The discovery of this completely new mechanism of gene regulation necessitated the development of a variety of techniques to further characterize miRNAs, their expression, and function. In this chapter, we will discuss techniques currently used in the miRNA field to detect, express and inhibit miRNAs, as well as methods used to identify and validate their targets, specifically with respect to the miRNAs encoded by human cytomegalovirus.

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Authors and Affiliations

  1. Vaccine and Gene Therapy Institute, Oregon Health and Sciences University, Beaverton, OR, USA

    Nicole L. Diggins, Lindsey B. Crawford, Hillary M. Struthers, Lauren M. Hook, Igor Landais, Rebecca L. Skalsky & Meaghan H. Hancock

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  1. Nicole L. Diggins
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  2. Lindsey B. Crawford
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  3. Hillary M. Struthers
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  4. Lauren M. Hook
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  5. Igor Landais
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  6. Rebecca L. Skalsky
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  7. Meaghan H. Hancock
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Correspondence to Meaghan H. Hancock .

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  1. Department of Microbiology and Immunology, Louisiana State University Health Sciences Center—Shreveport, Shreveport, LA, USA

    Andrew D. Yurochko

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Diggins, N.L. et al. (2021). Techniques for Characterizing Cytomegalovirus-Encoded miRNAs. In: Yurochko, A.D. (eds) Human Cytomegaloviruses. Methods in Molecular Biology, vol 2244. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1111-1_16

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  • DOI: https://doi.org/10.1007/978-1-0716-1111-1_16

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