Extraction of Nematodes from Plant Materials

Abstract

Plant parasitic and nonparasitic nematodes are present in leaves, stems, seeds, roots, tubers, and bulbs. Nematode extraction methods from plant materials are based on mobility of nematodes. Extraction methods include Funnel spray method, Dissection method, Blender nematode filter method, funnel spray method, and blender centrifugal flotation method. Dissection method is a direct method that can be directly used for microscopy with desired staining technique. Inactive nematodes can be isolated by the blender centrifugal flotation method.