Abstract
During the electroporation of T. reesei, linearized exogenous DNA is absorbed into swollen conidia by an electrical impulse. The advantage of this method is that it is less time-consuming, less expensive, and easier to perform than the classical protoplast transformation while at the same time having a comparable efficiency.
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References
Neumann E, Schaefer-Ridder M, Wang Y, Hofschneider PH (1982) Gene transfer into mouse lyoma cells by electroporation in high electric fields. EMBO J 1(7):841–845
Bowyer P (2001) DNA-mediated transformation of fungi. Oxford University Press, Oxford, pp 33–46
Sànchez O, Aguirre J (1996) Efficient transformation of Aspergillus nidulans by electroporation of germinated conidia. Fungal Genet Rep 43(21):48–51. https://doi.org/10.4148/1941-4765.1317
Schuster A, Bruno KS, Collett JR, Baker SE, Seiboth B, Kubicek CP, Schmoll M (2012) A versatile toolkit for high throughput functional genomics with Trichoderma reesei. Biotechnol Biofuels 5(1):1. https://doi.org/10.1186/1754-6834-5-1
Kim S, Miasnikov A (2013) Method for introducing nucleic acids into fungal cells. US Patent application US 2010/0304468 A1, 23 Aug 2010
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Wanka, F. (2021). Open the Pores: Electroporation for the Transformation of Trichoderma reesei. In: Mach-Aigner, A.R., Martzy, R. (eds) Trichoderma reesei. Methods in Molecular Biology, vol 2234. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1048-0_6
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DOI: https://doi.org/10.1007/978-1-0716-1048-0_6
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