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Molecular Identification of Trichoderma reesei

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Trichoderma reesei

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2234))

Abstract

Fungi comprise one of the most diverse groups of eukaryotes with many cryptic species that are difficult to identify. In this chapter, we detail a protocol for the molecular identification of the most industrially relevant species of Trichoderma—T. reesei. We first describe how a single spore culture should be isolated and used for the sequencing of the diagnostic fragment of the tef1 gene. Then, we provide two alternative methods that can be used for molecular identification and offer the diagnostic oligonucleotide hallmark of the tef1 sequence that is present in sequences of all T. reesei strains known to date and that is therefore suitable for reliable and straightforward identification.

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Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (KJQN201920), the Ministry of Science and Technology of Jiangsu Province (BK20180533), China, and the Postdoctoral Science Foundation (198162), all to FC. The work in Vienna (Austria) was supported by the Austrian Science Fund (FWF) P25613-B20 and P25745-B20, to ISD, and the Vienna Science and Technology Fund (WWTF), LS13-048, to ISD.

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Authors and Affiliations

  1. Fungal Genomics Laboratory (FungiG), The Key Laboratory of Plant Immunity, Nanjing Agricultural University, Nanjing, China

    Mohammad J. Rahimi, Feng Cai & Irina S. Druzhinina

  2. Institute of Chemical, Environmental and Bioscience Engineering (ICEBE), TU Wien, Vienna, Austria

    Mohammad J. Rahimi, Marica Grujic, Komal Chenthamara & Irina S. Druzhinina

Authors
  1. Mohammad J. Rahimi
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  2. Feng Cai
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  3. Marica Grujic
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  4. Komal Chenthamara
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  5. Irina S. Druzhinina
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Corresponding author

Correspondence to Irina S. Druzhinina .

Editor information

Editors and Affiliations

  1. Institute of Chemical, Environmental and Bioscience Engineering, TU Wien, Vienna, Austria

    Astrid R. Mach-Aigner

  2. Christian Doppler Laboratory for Optimized Expression of Carbohydrate-Active Enzymes, Institute of Chemical, Environmental and Bioscience Engineering, TU Wien, Vienna, Austria

    Roland Martzy

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Rahimi, M.J., Cai, F., Grujic, M., Chenthamara, K., Druzhinina, I.S. (2021). Molecular Identification of Trichoderma reesei. In: Mach-Aigner, A.R., Martzy, R. (eds) Trichoderma reesei. Methods in Molecular Biology, vol 2234. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1048-0_14

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  • DOI: https://doi.org/10.1007/978-1-0716-1048-0_14

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1047-3

  • Online ISBN: 978-1-0716-1048-0

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