Abstract
(R)-2-hydroxyglutarate (R-2-hg) has gained significant interest as a circulating biomarker for patients with cancers that harbor mutations in the isocitrate dehydrogenase (IDH) gene. R-2-hg detection is challenging as it must be separated from its enantiomer, (S)-2-hydroxyglutarate (S-2-hg). Here, we present a novel gas chromatography–tandem mass spectrometry method (GC-MS/MS) assay that can be used to separate and detect 2-hg enantiomers for clinical use.
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Acknowledgments
This work was supported by The Shimadzu Corporation and The Head for The Cure Foundation. SKS was supported by The Institute for Translational Sciences at The University of Texas Medical Branch, supported, in part, by a Clinical and Translational Science Award NRSA (TL1) Training Core (TL1TR001440) from the National Center for Advancing Translational Sciences, National Institutes of Health.
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Strain, S.K., Groves, M.D., Emmett, M.R. (2021). Enantioseparation and Detection of (R)-2-Hydroxyglutarate and (S)-2-Hydroxyglutarate by Chiral Gas Chromatography–Triple Quadrupole Mass Spectrometry. In: Wood, P.L. (eds) Metabolomics . Neuromethods, vol 159. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0864-7_8
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DOI: https://doi.org/10.1007/978-1-0716-0864-7_8
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