Abstract
Intravital imaging on live animals has provided new insights into the dynamics of tumor cells within their orthotopic microenvironment. In this chapter, we present a detailed method for intravital imaging of glioblastoma (GBM) cells in the mouse brain, with particular emphasis on the interactions of GBM cells with the surrounding vasculature.
This method involves the implantation of a cranial window and longitudinal intravital imaging as well as the analysis of tumor cells within their orthotopic microenvironment in vivo at a single-cell resolution using multiphoton imaging.
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Acknowledgments
We thank Pauline Deshors, Aafrin Pettiwala, and Guillaume Bourmeau (Institut Curie Research Center, Orsay-Paris) for critical reading and discussion. A special thank goes to Renaud Chabrier for illustration at Fig. 4.1. This work was supported by the Fondation ARC pour la recherche sur le cancer, the Inserm-CNRS ATIP-Avenir grant, the European Research Council (ERC) under the European Union’s Horizon 2020 (grant agreement no. 805225), and the NanoTheRad grant from Paris-Saclay University.
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Pichol-Thievend, C., Julien, B., Anézo, O., Philip, B., Seano, G. (2021). Intravital Imaging of Brain Tumors. In: Seano, G. (eds) Brain Tumors. Neuromethods, vol 158. Springer, New York, NY. https://doi.org/10.1007/978-1-0716-0856-2_4
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DOI: https://doi.org/10.1007/978-1-0716-0856-2_4
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