Abstract
Chromosome conformation capture and its variants have allowed chromatin topology to be interrogated at a superior resolution and throughput than by microscopic methods. Among the method derivatives, 4C-seq (circular chromosome conformation capture, coupled to high-throughput sequencing) is a versatile, cost-effective means of assessing all chromatin interactions with a specific genomic region of interest, making it particularly suitable for interrogating chromatin looping events. We present the principles and procedures for designing and implementing successful 4C-seq experiments.
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Karasu, N., Sexton, T. (2021). 4C-Seq: Interrogating Chromatin Looping with Circular Chromosome Conformation Capture. In: Bodega, B., Lanzuolo, C. (eds) Capturing Chromosome Conformation. Methods in Molecular Biology, vol 2157. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0664-3_3
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DOI: https://doi.org/10.1007/978-1-0716-0664-3_3
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Publisher Name: Humana, New York, NY
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