Abstract
The tegument (outer surface) of Schistosoma mansoni and other trematodes is in intimate contact with the host and plays an important role in host-parasite interactions. It is a complex structure that contains hundreds of proteins implicated in a variety of functions, although, so far, only a few proteins have been well characterized. Indeed, a few of these proteins have been shown to be effective vaccine and diagnostic candidates against S. mansoni and other schistosomes, and so the proteomic characterization of tegumental molecules could open new avenues for the development of novel control and surveillance strategies to combat schistosomiasis. Here, we describe the step by step isolation of tegumental proteins from the different tegument compartments using a biotinylation approach, as well as the materials and reagents needed.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Jones MK, Gobert GN, Zhang L, Sunderland P, McManus DP (2004) The cytoskeleton and motor proteins of human schistosomes and their roles in surface maintenance and host–parasite interactions. BioEssays 26(7):752–765
Ramajo-Hernández A, Pérez-Sánchez R, Ramajo-Martín V, Oleaga A (2007) Schistosoma bovis: plasminogen binding in adults and the identification of plasminogen-binding proteins from the worm tegument. Exp Parasitol 115(1):83–91
Skelly PJ, Da’dara AA, Li X-H, Castro-Borges W, Wilson RA (2014) Schistosome feeding and regurgitation. PLoS Pathog 10(8):e1004246
Skelly PJ, Alan WR (2006) Making sense of the schistosome surface. Adv Parasitol 63:185–284
Braschi S, Wilson RA (2006) Proteins exposed at the adult schistosome surface revealed by biotinylation. Mol Cell Proteomics 5(2):347–356
Fonseca CT, Braz Figueiredo Carvalho G, Carvalho Alves C, de Melo TT (2012) Schistosoma tegument proteins in vaccine and diagnosis development: an update. J Parasitol Res 2012:54126
Wilson RA (2012) Proteomics at the schistosome-mammalian host interface: any prospects for diagnostics or vaccines? Parasitology 139(9):1178–1194
Zhang M, Fu Z, Li C, Han Y, Cao X, Han H et al (2015) Screening diagnostic candidates for schistosomiasis from tegument proteins of adult Schistosoma japonicum using an immunoproteomic approach. PLoS Negl Trop Dis 9(2):e0003454
ClinicalTrials.gov. A phase I study of the safety, reactogenicity, and immunogenicity of Sm-TSP-2/alhydrogel® with or without GLA-AF for intestinal schistosomiasis in healthy adults
Hockley D, McLaren D (1971) The outer membrane of Schistosoma mansoni. Trans R Soc Trop Med Hyg 65(4):432
Mulvenna J, Moertel L, Jones MK, Nawaratna S, Lovas EM, Gobert GN et al (2010) Exposed proteins of the Schistosoma japonicum tegument. Int J Parasitol 40(5):543–554
Sotillo J, Pearson M, Becker L, Mulvenna J, Loukas A (2015) A quantitative proteomic analysis of the tegumental proteins from Schistosoma mansoni schistosomula reveals novel potential therapeutic targets. Int J Parasitol 45(8):505–516
Acknowledgments
This work was supported by a grant from the Health Institute Carlos III (ISCIII), Ministry of Economy and Competitiveness (Spain) (MPY 406/18), and a Miguel Servet I fellowship to JS (CP17III/00002) and a program grant (APP# 1037304) from the National Health and Medical Research Council (NHMRC, Australia) and a Senior Principal Research fellowship from NHMRC to AL (APP# 1117504).
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2020 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Pearson, M.S., Loukas, A., Sotillo, J. (2020). Proteomic Analysis of Schistosoma mansoni Tegumental Proteins. In: Timson, D.J. (eds) Schistosoma mansoni. Methods in Molecular Biology, vol 2151. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0635-3_8
Download citation
DOI: https://doi.org/10.1007/978-1-0716-0635-3_8
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-0634-6
Online ISBN: 978-1-0716-0635-3
eBook Packages: Springer Protocols