Abstract
Individual developmental stages of blood fluke Schistosoma mansoni excrete or secrete a different set of molecules. Here we describe optimized protocols for collection of excretory/secretory products (E/S products) from cercariae, schistosomula, adult worms, and eggs. These E/S products are essential for successful parasitism functioning at the host-parasite interface, enabling invasion into the host and contributing to the survival of the parasite by modulation of host physiology and immune responses. Collection of sufficient amounts of E/S products is required for detailed research of these processes.
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Acknowledgments
This work was supported by the project 1404317 from the Charles University Grant Agency; grants 19-17269S and 18-14167S from the Czech Science Foundation; Operational Programme Research, Development, and Education; the Call International Mobility of Researchers—MSCA—IF CZ.02.2.69/0.0/0.0/17_050/0008014; and the institutional project RVO 61388963.
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Leontovyč, A., Ulrychová, L., Horn, M., Dvořák, J. (2020). Collection of Excretory/Secretory Products from Individual Developmental Stages of the Blood Fluke Schistosoma mansoni. In: Timson, D.J. (eds) Schistosoma mansoni. Methods in Molecular Biology, vol 2151. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0635-3_5
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DOI: https://doi.org/10.1007/978-1-0716-0635-3_5
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