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Viral Transduction of DRG Neurons

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Axon Degeneration

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2143))

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Abstract

The manipulation of gene expression is an essential tool to study the function of genes or signaling pathways. Uniform and robust gene manipulation is crucial for successful assays. However, neuronal cells are generally difficult-to-transfect cells with conventional DNA/RNA transfection reagents. Therefore, virus-mediated gene delivery is a primary choice for the studies of gene functions in neurons. In this chapter, we will describe the methods for lentivirus-mediated gene expression or knockdown in DRG neurons.

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Acknowledgments

This work was supported by the National Institutes of Health (Grant RO1AG013730 (Jeffrey Milbrandt: J.M.); RO1NS065053 and RO1NS087632 (J.M. and Aaron DiAntonio: A.D.)). The author thanks J.M., A.D., and members of the J.M. and A.D.’s laboratories for fruitful discussions.

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Authors and Affiliations

  1. Department of Genetics, Washington University in St. Louis, Couch Biomedical Research Building, St. Louis, MO, USA

    Yo Sasaki

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  1. Yo Sasaki
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Correspondence to Yo Sasaki .

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Editors and Affiliations

  1. Department of Biochemistry, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo, Buffalo, NY, USA

    Elisabetta Babetto

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Sasaki, Y. (2020). Viral Transduction of DRG Neurons. In: Babetto, E. (eds) Axon Degeneration. Methods in Molecular Biology, vol 2143. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0585-1_5

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  • DOI: https://doi.org/10.1007/978-1-0716-0585-1_5

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0584-4

  • Online ISBN: 978-1-0716-0585-1

  • eBook Packages: Springer Protocols

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