Abstract
Macrophages are phagocytic cells that constitute the primary barrier against pathogens. After phagocytosis a single-membraned vesicle that contains the pathogen is formed. This phagosome undergoes a maturation process to acquire an increasingly antimicrobial environment. Leptospiral uptake by macrophages induces the formation of a Leptospira-containing phagosome (LCP). The kinetics of lysosomal marker recruitment by the LCP is correlated with virulence. This chapter presents a protocol to study the intracellular trafficking of Leptospira spp. within macrophages by fluorescent labeling bacteria and different markers of the phagocytic pathway. We also describe a method to evaluate the bacterial survival within macrophages.
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Acknowledgments
This work was supported by JSPS KAKENHI grant JP21590484 (C.T.).
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Toma, C., Suzuki, T. (2020). Evaluation of Intracellular Trafficking in Macrophages. In: Koizumi, N., Picardeau, M. (eds) Leptospira spp.. Methods in Molecular Biology, vol 2134. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0459-5_18
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DOI: https://doi.org/10.1007/978-1-0716-0459-5_18
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-0459-5
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