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Ligand Fishing: An Approach for the Discovery of Inhibitors from Complex Biological Mixtures

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Targeting Enzymes for Pharmaceutical Development

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2089))

Abstract

Ligand fishing is a convenient bioanalytical screening method that is based on the affinity selection of a ligand from a complex biological sample by an immobilized receptor. It is a versatile affinity-based screening approach and it has found application in multiple interacting pairs such as enzyme-inhibitor/activator, antigen-antibody, receptor-ligand, and protein-protein. Important parameters that affect the successful operation of the method are the high specificity and strong binding affinity of the interacting pair (e.g., enzyme-ligand complex) and the elution of the bound ligand from the complex. This chapter provides protocols for the synthesis of affinity adsorbent and its application in off-line ligand-fishing procedure for a 6His-tagged glutathione transferase (GST).

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Acknowledgments

E.G.C. thanks IKY Scholarship Programs that is co-financed by Greece and the European Union (European Social Fund- ESF) through the Operational Programme «Human Resources Development, Education and Lifelong Learning» in the context of the project “Reinforcement of Postdoctoral Researchers” (MIS-5001552), implemented by the State Scholarships Foundation (IKY).

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Authors and Affiliations

  1. Laboratory of Enzyme Technology, Department of Biotechnology, School of Applied Biology and Biotechnology, Agricultural University of Athens, Athens, Greece

    Evangelia G. Chronopoulou, Christina Varotsou, Nikolaos Georgakis, Georgios Premetis, Elisavet Ioannou & Nikolaos E. Labrou

Authors
  1. Evangelia G. Chronopoulou
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  2. Christina Varotsou
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  3. Nikolaos Georgakis
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  4. Georgios Premetis
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  5. Elisavet Ioannou
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  6. Nikolaos E. Labrou
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Corresponding author

Correspondence to Nikolaos E. Labrou .

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Editors and Affiliations

  1. Department of Biotechnology, Agricultural University of Athens, Athens, Greece

    Nikolaos E. Labrou

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Chronopoulou, E.G., Varotsou, C., Georgakis, N., Premetis, G., Ioannou, E., Labrou, N.E. (2020). Ligand Fishing: An Approach for the Discovery of Inhibitors from Complex Biological Mixtures. In: Labrou, N. (eds) Targeting Enzymes for Pharmaceutical Development. Methods in Molecular Biology, vol 2089. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0163-1_15

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  • DOI: https://doi.org/10.1007/978-1-0716-0163-1_15

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0162-4

  • Online ISBN: 978-1-0716-0163-1

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