Abstract
Induced pluripotent stem cells (iPSCs) were originally derived from adult somatic cells by ectopic expression of the stem cell transcription factors OCT3/4, SOX2, c-Myc, and KLF4. The characteristic features of iPSCs are similar to those of embryonic stem cells; they can be expanded indefinitely in vitro and differentiated into the three germ layers: endoderm, mesoderm, and ectoderm. The breakthrough discovery by Takahashi and Yamanaka that somatic cells can be “reprogrammed” to generate iPSCs has led to extensive use of iPSCs and their differentiated cells thereof, in diverse research areas, such as regenerative medicine, development, as well as establishment of disease-specific models, thus providing the platform for personalized patient-specific medicine.
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Abbreviations
- EB:
-
Embryoid body
- FBS:
-
Fetal bovine serum
- hESC:
-
Human embryonic stem cells
- iPSC:
-
Induced pluripotent stem cells
- KLF4:
-
Kruppel-like factor-4
- OCT3/4:
-
Octamer-binding transcription factor-3/4
- PBS:
-
Phosphate buffered saline
- SOX2:
-
SRY (sex determining region Y)-box 2
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Acknowledgments
This work was supported by the Rappaport Research Institute [#01012020RI]; the Israel Science Foundation (ISF) [# 824/19]; the Niedersachsen Foundation [#ZN3452]; and the US-Israel Binational Foundation (BSF) [#2019039].
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Neeman-Egozi, S., Baskin, P., Binah, O. (2021). Methods for Isolation and Reprogramming of Various Somatic Cell Sources into iPSCs. In: Nagy, A., Turksen, K. (eds) Induced Pluripotent Stem (iPS) Cells. Methods in Molecular Biology, vol 2454. Humana, New York, NY. https://doi.org/10.1007/7651_2021_387
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DOI: https://doi.org/10.1007/7651_2021_387
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