Skip to main content

Method for Evaluating Neuromodulatory Properties of Dental Pulp Stem Cell as an In Vitro Model for Parkinson’s Disease

  • Protocol
  • First Online:
Stem Cell Nanotechnology

Abstract

Several research groups have utilized dental pulp stem cells for numerous studies as treatment modality for Parkinson’s disease (PD). However, the roles of dental pulp stem cells in governing the Parkinson’s disease inflammatory microenvironment remain to be evaluated. In this article, we elaborate the method where we can investigate the effects of dental pulp stem cells on neurons and microglia in an in vitro inflammatory microenvironment.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Similar content being viewed by others

References

  1. Walker L, Stefanis L, Attems J (2019) Clinical and neuropathological differences between Parkinson’s disease, Parkinson’s disease dementia and dementia with Lewy bodies—current issues and future directions. J Neurochem. https://doi.org/10.1111/jnc.14698

    Article  CAS  Google Scholar 

  2. Parihar A, Parihar P, Solanki I, Parihar MS (2019) Alpha synuclein and Parkinson’s disease. In: Pathology, prevention and therapeutics of neurodegenerative disease. Springer, Singapore, pp 1–14

    Google Scholar 

  3. Palmer C, Coronel R, Bernabeu-Zornoza A, Liste I (2019) Therapeutic application of stem cell and gene therapy in Parkinson’s disease. In: Pathology, prevention and therapeutics of neurodegenerative disease. Springer, Singapore, pp 159–171

    Chapter  Google Scholar 

  4. Abu Kasim NH, Govindasamy V, Gnanasegaran N, Musa S, Pradeep PJ, Srijaya TC, Aziz ZACA (2015) Unique molecular signatures influencing the biological function and fate of post-natal stem cells isolated from different sources. J Tissue Eng Regen Med 9(12):E252–E266

    Article  CAS  Google Scholar 

  5. Jayaraman P, Govindasamy V, Gnanasegaran N, Kunasekaran W, Vasanthan P, Musa S, Kasim NHA (2016) Expression patterns of immune genes in long-term cultured dental stem cells. Clin Oral Investig 20(1):109–116

    Article  Google Scholar 

  6. Gnanasegaran N (2019) Myriad applications of dental pulp stem cells. Arq Bras Oftalmol 82(3):258

    Article  Google Scholar 

  7. Gnanasegaran N, Govindasamy V, Musa S, Kasim NHA (2016) ReNCell VM conditioned medium enhances the induction of dental pulp stem cells into dopaminergic like cells. Cytotechnology 68(2):343–353

    Article  CAS  Google Scholar 

  8. Gnanasegaran N, Govindasamy V, Kathirvaloo P, Musa S, Abu Kasim NH (2018) Effects of cell cycle phases on the induction of dental pulp stem cells toward dopaminergic-like cells. J Tissue Eng Regen Med 12(2):e881–e893

    Article  CAS  Google Scholar 

  9. Gnanasegaran N, Govindasamy V, Mani V, Abu Kasim NH (2017) Neuroimmunomodulatory properties of DPSCs in an in vitro model of Parkinson’s disease. IUBMB Life 69(9):689–699

    Article  CAS  Google Scholar 

  10. Bindal P, Ramasamy TS, Kasim NHA, Gnanasegaran N, Chai WL (2018) Immune responses of human dental pulp stem cells in lipopolysaccharide-induced microenvironment. Cell Biol Int 42(7):832–840

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by University of Malaya, Kuala Lumpur, Malaysia. Authors thank scientific input and relevant discussions from Envirotransgene® Global, Chennai, India.

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2019 Springer Science+Business Media New York

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Gnanasegaran, N., Thimiri Govinda Raj, D.B., Arumugam, S. (2019). Method for Evaluating Neuromodulatory Properties of Dental Pulp Stem Cell as an In Vitro Model for Parkinson’s Disease. In: Turksen, K. (eds) Stem Cell Nanotechnology. Methods in Molecular Biology, vol 2125. Humana, New York, NY. https://doi.org/10.1007/7651_2019_261

Download citation

  • DOI: https://doi.org/10.1007/7651_2019_261

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0359-8

  • Online ISBN: 978-1-0716-0360-4

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics