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Liver Bioengineering Using Decellularized Whole-Liver Scaffolds

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Decellularized Scaffolds and Organogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1577))

Abstract

Currently, due to the progress made in the field of regenerative medicine, whole-organ bioengineering is becoming a valid alternative to cope with the shortages of organs for transplantation. In this chapter, we describe the main techniques carried out for pig liver bioengineering, which serves as an essential model for future human liver bioengineering. These include porcine whole-liver decellularization, endothelial and mesenchymal stem cell isolation, porcine ES-derived hepatoblasts, and scaffold recellularization using a bioreactor perfusion system.

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References

  1. Baptista PM et al (2011) The use of whole organ decellularization for the generation of a vascularized liver organoid. Hepatology 53:604–617

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Acknowledgments

This work was supported by Instituto de Salud Carlos III, through a predoctoral fellowship i-PFIS IFI15/00158 (I. P-P), Gobierno de Aragón and Fondo Social Europeo through a predoctoral fellowship DGA C066/2014 (P. S-A), and by a predoctoral fellowship from Fundação para Ciência e Tecnologia (PD/BD/114057/2015), Portugal (S.M.). PMB was supported by an H2020-MSCA-IF-2014 from the European Research Agency and by the PI15/00563 Research Project from Instituto de Salud Carlos III, Madrid, Spain.

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Authors and Affiliations

  1. Instituto de Investigación Sanitaria de Aragón (IIS Aragón), Zaragoza, Spain

    Iris Pla-Palacín, Pilar Sainz-Arnal, Sara Morini, Manuel Almeida & Pedro M. Baptista

  2. Instituto Aragonés de Ciencias de la Salud (IACS), Zaragoza, Spain

    Pilar Sainz-Arnal

  3. Department of Bioengineering and iBB, Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Lisboa, Portugal

    Sara Morini

  4. Center for Biomedical Research Network Liver and Digestive Diseases (CIBERehd), Zaragoza, Spain

    Pedro M. Baptista

  5. Instituto de Investigación Sanitaria de la Fundación Jiménez Díaz, Madrid, Spain

    Pedro M. Baptista

  6. Biomedical and Aerospace Engineering Department, Universidad Carlos III de Madrid, Madrid, Spain

    Pedro M. Baptista

Authors
  1. Iris Pla-Palacín
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  2. Pilar Sainz-Arnal
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  3. Sara Morini
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  4. Manuel Almeida
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  5. Pedro M. Baptista
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Corresponding author

Correspondence to Pedro M. Baptista .

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Editors and Affiliations

  1. Ottawa Hospital Research Institute, Ottawa, ON, Canada

    Kursad Turksen

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© 2017 Springer Science+Business Media New York

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Pla-Palacín, I., Sainz-Arnal, P., Morini, S., Almeida, M., Baptista, P.M. (2017). Liver Bioengineering Using Decellularized Whole-Liver Scaffolds. In: Turksen, K. (eds) Decellularized Scaffolds and Organogenesis. Methods in Molecular Biology, vol 1577. Humana Press, New York, NY. https://doi.org/10.1007/7651_2017_98

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  • DOI: https://doi.org/10.1007/7651_2017_98

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7655-3

  • Online ISBN: 978-1-4939-7656-0

  • eBook Packages: Springer Protocols

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